Welcome to MIssense variant to protein StruCture Analysis web SuiTe



MISCAST is developed at the Broad Institute of MIT and Harvard, by a combined effort from the Genetics and Therapeutics group of Stanley Center. The goal of MISCAST is to visualize and analyze single amino-acid-altering missense variants on protein sequence and 3-dimensional structure, and thereby forecast their biological impact.

The website provides two primary tracks. The Variant Summary Report track provides an amino-acid-wise report, summarizing its pathogenic and population variant-associated protein features. The Variant Analysis Suite provides a platform for detailed exploration, analysis, and visualization of protein features and missense variants on 1D, 2D and 3D protein structure space.

Disclaimer: Please note that all content on this website is provided for academic and research purposes only. It has not undergone clinical validation, and should not be used for medical advice, diagnosis, or treatment. For best experience, please open Variant Analysis Suite on a laptop/desktop instead of any smaller screen.


Copyright © Broad Institute. All rights reserved.

Select Gene:





   Disclaimer: Please note that all content on this website is provided for academic and research purposes only. It has not undergone clinical validation, and should not be used for medical advice, diagnosis, or treatment.

Explore Protein Features of Missense Variants by Gene







  3-class Secondary Structure


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  8-class Secondary Structure


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  Accessible Surface Area


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  Twenty Amino Acids and Physiochemical Property


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  Protein-protein Interaction types

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  Distance to Post-translational Modification (PTM) Types


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  UniProt-based Functional Features

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  3-class Secondary Structure

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  8-class Secondary Structure

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  Acessible Surface Area/Residue Exposure

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  Amino Acids and Physiochemical Property

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  Protein-protein Interactions

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  Post-translational Modifications (PTMs)

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  UniProt-based Functional Features

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