is an online resource developed at the Stanley Center
in the Broad Institute
of MIT and Harvard, by a combined effort from the Genetics and Therapeutics group. The goal of MISCAST is to interpret single amino-acid-altering missense variants on protein 3-dimensional structure and to thereby forecast their biological impact.
The dataset provided on this website spans 1,330 human genes, including 406,449 population variants from gnomAD
(release 2.1.1), 54,137 pathogenic variants and disease mutations from the ClinVar
(February, 2019 release) and HGMD
, (Professional release 2018.4) databases, respectively, and >14k molecularly-solved human protein 3D structures from the Protein Data Bank (PDB
). The website provides two separate tracks. The Variant Summary Reports
track provides an amino-acid-wise report, summarizing its pathogenic and population variant-associated protein features. The Variant Analysis Suite
provides a platform for detailed exploration, analysis, and visualization of protein features and missense variants on 1D, 2D and 3D protein structure space. Disclaimer
: Please note that all content on this website is provided for academic and research purposes only. It has not undergone clinical validation, and should not be used for medical advice, diagnosis, or treatment. For best experience, please open Variant Analysis Suite on a laptop/desktop instead of any smaller screen.